Šupe-Domić, Daniela and Milas, Goran and Stanišić, Lada and Drmić Hofman, Irena and Martinović Klarić, Irena (2018) Reference intervals for six salivary cortisol measures based on the Croatian Late Adolescence Stress Study (CLASS). Biochemia medica, 28 (1). pp. 1-4. ISSN 1330-0962 (Print), 1846-7482 (Online)
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BM 2018_1 Šupe-Domić, Milas, Stanišić, Drmić Hofman, Martinović Klarić.pdf Download (816Kb) | Preview |
Abstract
IN ENGLISH: INTRODUCTION: The aim of this nested study is to provide the reference intervals for already published measurements of salivary cortisol from the Croatian Adolescence Stress Study (CLASS). MATERIAL AND METHODS: A total of 969 individuals (372 males and 597 females) were included in the reference sample (age range: 18-21 years). Salivary cortisol concentrations were determined by the enzyme immunoassay (LUCIO-Medical ELISA Salivary Cortisol Kit, Nal von Minden, Germany) in the Department of Medical Laboratory Diagnostics, University Hospital Split. Nonparametric statistics were used for calculating the reference intervals (RIs) and 90% confidence intervals (90% CIs). RESULTS: The lower limits of RIs determined by the direct method were higher in females (> 10%) than in males for the cortisol concentrations at awakening (SCC0), 30 to 45 after awakening (SCC30-45) and at bedtime (SCCbedtime). The upper limits of RIs for the SCCbedtime were higher (> 10%) in males than in females. Females also had higher upper limits of RIs for the cortisol awakening response (CAR) and the diurnal cortisol slope (DCS) and higher lower limits of RIs for the CAR and the area under the curve with respect to ground (AUCG). The lower limits of RIs for the DCS were higher in males than in females. CONCLUSIONS: Obtained reference values open the arena for introducing salivary bioscience in Croatian clinical laboratory practice and provide important data for better understanding of gender differences in adaptation to stress during late adolescence. --------------- IN CROATIAN: UVOD: Cilj ove studije bio je definirati referentne intervale za objavljene mjere salivarnog kortizola iz Hrvatskog istraživanja stresa u kasnoj adolescenciji (CLASS). MATERIJALI I METODE: Ukupno 969 ispitanika (372 djevojaka i 597 mladića) uključeno je u referentni uzorak (18-21 godine starosti). Koncentracije salivarnog kortizola izmjerene su imunokemijskom ELISA metodom (LUCIO-Medical ELISA Salivary Cortisol Kit, Nal von Minden, Germany) u Zavodu za medicinsko laboratorijsku dijagnostiku Kliničkog bolničkog centra Split. Referentni intervali (RI) i 90%-tni intervali pouzdanosti (90% CI) izračunati su neparametrijskim metodama. REZULTATI: Donja granica referentnog intervala određena izravnom metodom veća je kod djevojaka (>10%) nego mladića za koncentracije kortizola u sve tri vremenske točke (po buđenju, 30-45 minuta nakon buđenja i neposredno prije lijeganja). Gornja granica referentnog intervala za koncentracije salivarnog kortizola neposredno prije lijeganja veća je kod mladića (>10%) u odnosu na djevojke. Također, djevojke imaju veću gornju granicu referentnog intervala za odgovor kortizola na buđenje (engl. cortisol awakening response, CAR) i cirkadijano smanjenje kortizola (engl. diurnal cortisol slope, DCS) te veću donju granicu referentnog intervala za CAR i površinu ispod krivulje u odnosu na nultu razinu (engl. area under the curve with respect to ground, AUCG). Indeks cirkadijanog smanjenja kortizola, DCS je u donjoj granici referentnog intervala veći kod mladića u odnosu na djevojke. ZAKLJUČCI: Objavljeni referentni intervali mjera salivarnog kortizola važni su zbog uvođenje salivarnih analiza u rutinsku kliničku praksu u RH i pridonose detaljnijim spoznajama o spolnim razlikama u adaptacijskim mehanizmima na stresne poticaje tijekom kasne adolescencije.
| Item Type: | Article |
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| Additional Information: | Language: English. - Title in Croatian: Referentni intervali šest mjera salivarnog kortizola u Hrvatskom istraživanju stresa u kasnoj adolescenciji (CLASS). |
| Uncontrolled Keywords: | Salivary cortisol, reference intervals, late adolescence (salivarni kortizol, referentni intervali, kasna adolescencija) |
| Subjects: | G Geography. Anthropology. Recreation > GN Anthropology |
| Depositing User: | Karolina |
| Date Deposited: | 06 Dec 2017 14:31 |
| Last Modified: | 06 Dec 2017 14:31 |
| URI: | http://idiprints.knjiznica.idi.hr/id/eprint/784 |
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